eight chamber cover glass slide (Fisher Scientific)

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Fisher Scientific eight chamber cover glass slide
Eight Chamber Cover Glass Slide, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glass+chamber+slides/glass+slide/pmc13276413-295-14-18
Average 86 stars, based on 1 article reviews

eight chamber cover glass slide - by Bioz Stars, 2026-09

86/100 stars

Images

Staining:

Article Title: Translational research into the effects of cigarette smoke on inflammatory mediators and epithelial TRPV1 in Crohn’s disease
Article Snippet: After this period, the integrity of the monolayer was evaluated by measuring the TEER using a Millicell ERS-2 Volt-Ohm Meter (Merck Millipore, Billerica, MA, USA) to ensure monolayers with TEER values of 700 Ω.cm 2 or higher were obtained [ ]. .. For TRPV1 staining, HT-29 and T-84 cells were plated on glass chamber slides (Fisher Scientific, Merelbeke, Belgium) at a cell density of 5 × 10 4 /well and cultured for 24 hours, after which the cells were harvested and washed with ice-cold PBS and fixed with 4% paraformaldehyde, while being kept in the chamber slides. ..

Article Title: Bioengineered intestinal muscularis complexes with long-term spontaneous and periodic contractions
Article Snippet: .. For staining of c-Kit, IMC were cultured on the glass chamber slides (Fisher Scientific, seeding density: 250,000 cells/chamber), fixed by acetone (Fisher Scientific) for 30 min at 4°C and permeabilized with 0.1% Triton X-100 in blocking solution. .. Images were taken by the Olympus IX71 or IX73 microscope with CellSens software.

Article Title: Bioengineered intestinal muscularis complexes with long-term spontaneous and periodic contractions.
Article Snippet: .. For staining of c-Kit, IMC were cultured on the glass chamber slides (Fisher Scientific, seeding density: 250,000 cells/chamber), fixed by acetone (Fisher Scientific) for 30 min at 4 ̊C and permeabilized with 0.1% Triton X-100 in blocking solution. .. Images were taken by the Olympus IX71 or IX73 microscope with CellSens software.

Cell Culture:

Article Title: Multichannel bridges and NSC synergize to enhance axon regeneration, myelination, synaptic reconnection, and recovery after SCI
Article Snippet: .. To investigate the effect of PLG bridge on hNSC differentiation in the presence of immune cues, hNSC were plated on either PLO/LAM coated PLG scaffolds or glass chamber slides (Fisher Scientific, Waltham, MA) and cultured for 14 DIV with DM, PMN-CM, or MØ-CM. hNSC differentiated on glass or PLG scaffolds were fixed with 4% paraformaldehyde at room temperature for 20 min. hNSC were permeabilized and blocked with 0.1% Triton X-100 (Sigma-Aldrich Inc, Saint Louis, MO) and 10% donkey serum (Jackson ImmunoResearch, West Grove, PA) in PBS. .. Next, hNSC were immunostained for neuronal marker Tubulinß III (Tubß III), astrocytic marker glial fibrillary acidic protein (GFAP), and oligodendrocyte marker (Olig2). hNSC were counterstained with nuclear dye Hoechst 33342 (1 μg/mL dilution; Invitrogen, Waltham, MA).

Article Title: Carnitine palmitoyltransferase-II inactivity promotes malignant progression of metabolic dysfunction-associated fatty liver disease via liver cancer stem cell activation
Article Snippet: .. T cells were cultured at 37 °C on glass chamber slides (Fisher Scientific, Leicestershire, United Kingdom), treated with bezafibrate for 24 hours, incubated for 15 minutes with 10 μM 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazolyl carbocyanine iodide fluorescent dye (JC-1, Molecular Probes, Paisley, United Kingdom) in the dark, washed according to the protocol of the manufacturer, and immediately imaged via a fluorescence microscope (Keyence, Osaka, Japan) with red (λ: 560 ± 40 nm, λ: 630 ± 60 nm) and green fluorescence (λ: 470 ± 40 nm, λ: 535 ± 50 nm)[ ]. .. Paraffin-embedded liver sections were dewaxed, rehydrated, subjected to antigen retrieval, incubated with 3% H 2 O 2 for 10 minutes, and then incubated with rabbit anti-mouse monoclonal CPT-II antibodies (Ab181114, diluted 1:80; Abcam, United Kingdom) at 4 °C overnight.

Article Title: Multichannel bridges and NSC synergize to enhance axon regeneration, myelination, synaptic reconnection, and recovery after SCI
Article Snippet: .. To investigate the effect of PLG bridge on hNSC differentiation in presence of immune cues, hNSC were plated on either PLO/LAM coated PLG scaffolds or glass chamber slides (Fisher Scientific, Waltham, MA) and cultured for 14 days in vitro (DIV) with DM, PMN-CM, or MØ-CM. hNSC differentiated on glass or PLG scaffolds were fixed with 4% paraformaldehyde at room temperature for 20 minutes. hNSC were permeabilized and blocked with 0.1% Triton X-100 (Sigma-Aldrich Inc, Saint Louis, MO) and 10% donkey serum (Jackson ImmunoResearch, West Grove, PA) in PBS. .. Next, hNSC were immunostained for neuronal marker Tubulinß III (Tubß III), astrocytic marker glial fibrillary acidic protein (GFAP), and oligodendrocyte marker Olig2. hNSC were counterstained with nuclear dye Hoechst 33342 (1 μg/mL dilution; Invitrogen, Waltham, MA).

Blocking Assay:

Confocal Laser Scanning Microscopy:

Article Title: Dually targeted bioinspired nanovesicle delays advanced prostate cancer tumour growth in vivo.
Article Snippet: Prostate cancer (PC) is second-leading cancer in men, with limited treatment options available for men with advanced and metastatic PC.. Prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA) have been exploited as therapeutic targets in PC due to their upregulation in the advanced stages of the disease.. To date, several PSAand PSMA-activatable prodrugs have been developed to reduce the systemic toxicity of existing chemotherapeutics.

Incubation:

Fluorescence:

Microscopy:

In Vitro:

Article Title: Multichannel bridges and NSC synergize to enhance axon regeneration, myelination, synaptic reconnection, and recovery after SCI
Article Snippet: .. To investigate the effect of PLG bridge on hNSC differentiation in presence of immune cues, hNSC were plated on either PLO/LAM coated PLG scaffolds or glass chamber slides (Fisher Scientific, Waltham, MA) and cultured for 14 days in vitro (DIV) with DM, PMN-CM, or MØ-CM. hNSC differentiated on glass or PLG scaffolds were fixed with 4% paraformaldehyde at room temperature for 20 minutes. hNSC were permeabilized and blocked with 0.1% Triton X-100 (Sigma-Aldrich Inc, Saint Louis, MO) and 10% donkey serum (Jackson ImmunoResearch, West Grove, PA) in PBS. .. Next, hNSC were immunostained for neuronal marker Tubulinß III (Tubß III), astrocytic marker glial fibrillary acidic protein (GFAP), and oligodendrocyte marker Olig2. hNSC were counterstained with nuclear dye Hoechst 33342 (1 μg/mL dilution; Invitrogen, Waltham, MA).

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